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Table 1 Transformation efficiencies of E2348/69 vs. E2348/69 ΔhsdR

From: Generation of a restriction minus enteropathogenic Escherichia coli E2348/69 strain that is efficiently transformed with large, low copy plasmids

PLASMID DESCRIPTION SIZE ORI COPY# TRANSFORMATION EFFICIENCIESa
      E2348/69b E2348/69c
pJW15 lux reporter ~10 kb p15A 10–12 2.5 × 10-11 8.3 × 10-5
pNLP10 lux reporter ~10 kb pSC101 ~5 0 5.4 × 10-5
pLAFR1 cosmid cloning vector 21.6 RK2 5–7 3.0 × 10-9 9.0 × 10-7
  1. a. Transformation efficiencies are expressed as the (number of transformants/number of viable cells)/ug DNA. All experiments were repeated at least three times, and one representative experiment is shown.
  2. b. Transformation efficiencies observed with plasmid isolated from E. coli K-12.
  3. c. Transformation efficiencies observed with plasmid isolated from E2348/69ΔhsdR.