Table 1 Conserved degenerate primers for the luxS and sahH gene. The luxS gene of Vibrio harveyi and the sahH gene of Rhodobacter sphaeroides served as the reference sequences for primer binding.

LuxS_degfor3

CAT TAT TAG ATA GCT TTA CAD TNG AYC AYA

30 bp

48

48°C

4

LuxS_degrev4

AGC GAG TGC ATC TGA TAA GWN CCR CAY TS

29 bp

64

48°C

410

LuxS_gammafor1

TRG ATA GCT TTA CHG TTG ACC ATA C

25 bp

6

48°C

11

LuxS_gammarev2

TAR AAR CCN GTR CGA CAT CCC AT

23 bp

32

48°C

263

SahH_degfor

GAS GAS ACN ACN ACN GGN GT

20 bp

1024

46°C

562

SahH_degrev

TCV DWR TCR AAR TGD CCR ATR TT

23 bp

1728

46°C

1009

SahH_alphafor

GCH GAR ACV GAR ATG CCS GGB YT

23 bp

432

60°C

64

SahH_alpharev

GAR CCY TTR CCS ACR TYR CCR WA

23 bp

512

60°C

788