|
Gene namea
|
University of Wisconsinb
|
TIGR
|
SANGER
|
Pump family or protein functionc
|
FCd
|
|---|
|
(floR)
|
y2173
|
NT02YP2579
|
YPO2148
|
MFS
|
12.7 ± 2.5
|
|
(yieO)
|
y0010
|
NT02YP0010
|
YPO0009
|
MFS
|
8.6 ± 1.1
|
|
(acrA*-acrB)
|
y3392*-y3393
|
NT02YP4041
|
YPO1000
|
RND
|
6.9 ± 0.4
|
|
acrA*-acrB
|
y1050*-y1049
|
NT02YP1227
|
YPO3132
|
RND
|
5.0 ± 0.4
|
|
(acrA*-acrB)
|
y3760*-y3759
|
NT02YP4463
|
YPO0420
|
RND
|
2.7 ± 0.1
|
|
-
|
y4041
|
NT02YP4789
|
YPO4020
|
DMT
|
1.7 ± 0.9
|
|
yegM*-yegN-yegO-yegB
|
y1386*-y1385-y1384-y1383
|
NT02YP1646
|
YPO2847
|
RND
|
1.4 ± 0.5
|
|
(ybjY*-ybjZ)
|
y2814*-y2813
|
NT02YP3366
|
YPO1364
|
ABC
|
1.4 ± 0.4
|
|
(acrA*-acrB)
|
y0702*-y0703
|
NT02YP0804
|
YPO3483
|
RND
|
1.3 ± 0.3
|
|
emrA*-emrB
|
y0922*-y0921
|
NT02YP1066
|
YPO3267
|
MFS
|
1.3 ± 0.4
|
|
(macA*-macB)
|
y1481*-y1480
|
NT02YP1756
|
YPO2999
|
RND
|
1.3 ± 0.5
|
|
ygeD
|
y3180
|
NT02YP3786
|
YPO0792
|
MFS
|
1.3 ± 0.2
|
|
fieF
|
y0060
|
NT02YP0067
|
YPO0077
|
CDF
|
1.2 ± 0.1
|
|
(abgT)
|
y3402
|
NT02YP4052
|
YPO1008
|
IT
|
1.1 ± 0.2
|
|
acrD
|
y1439
|
NT02YP1705
|
YPO3043
|
RND
|
1.1 ± 0.1
|
|
sugE
|
y0613
|
NT02YP0702
|
YPO0355
|
SMR
|
1.1 ± 0.4
|
|
(ynfM)
|
y2108
|
NT02YP2497
|
YPO2266
|
MFS
|
1.1 ± 0.4
|
|
bcr
|
y2916
|
NT02YP3488
|
YPO1267
|
MFS
|
1.1 ± 0.3
|
|
aaeA*-aaeB
|
y0178*-y0177
|
NT02YP0192
|
YPO3685
|
ArAE
|
1.1 ± 0.0
|
|
(yjcR*-Q)
|
y3558*-y3559
|
NT02YP4231
|
YPO0619
|
RND
|
1.0 ± 0.2
|
|
yajR
|
y1017
|
NT02YP1187
|
YPO3169
|
MFS
|
1.0 ± 0.1
|
|
arsB
|
y0844
|
NT02YP0968
|
YPO3347
|
IT
|
1.0 ± 0.4
|
|
corC
|
y1191
|
NT02YP1397
|
YPO2617
|
HCC
|
0.9 ± 0.1
|
|
mdtJ*-mdtI
|
y2242*-y2241
|
NT02YP2670
|
YPO2068
|
SMR
|
0.8 ± 0.2
|
|
rosA
|
y1087
|
NT02YP1268
|
YPO3093
|
MFS
|
0.8 ± 0.1
|
|
ydhC
|
y1948
|
NT02YP2306
|
YPO2389
|
MFS
|
0.8 ± 0.2
|
| |
y3186
|
NT02YP3792
|
YPO0798
|
MFS
|
0.7 ± 0.1
|
|
(ydhE)
|
y1945
|
NT02YP2302
|
YPO2392
|
MATE
|
0.7 ± 0.1
|
|
(ybeQ)
|
y1874
|
NT02YP2220
|
YPO1712
|
MFS
|
0.7 ± 0.0
|
|
mdlA*-mdlB
|
y1039*-y1040
|
NT02YP1213
|
YPO3145
|
ABC
|
0.7 ± 0.0
|
|
ydeF
|
y2653
|
NT02YP3168
|
YPO1515
|
MFS
|
0.5 ± 0.1
|
|
emrE, gacE
|
y2000
|
NT02YP2368
|
YPO2333
|
SMR
|
0.5 ± 0.0
|
|
mdfA, cmr
|
y4067
|
NT02YP4824
|
YPO4048
|
MFS
|
0.2 ± 0.0
|
|
(ydjV)
|
y2272
|
NT02YP2703
|
YPO2040
|
MFS
|
nd
|
- a Gene name as annotated for Y. pestis strain KIM and/or CO92 or gene names (in parentheses) given herein based on the name of their E. coli homologs. Multiple names for the same gene are separated by commas. Genes of multi-component pumps are separated by dashes. The star (*) marks genes from multi-component pump gene clusters whose transcripts were analyzed by qRT-PCR. b Gene designations in the University of Wisconsin, TIGR, and SANGER Y. pestis genome databases. The Wisconsin column shows designations for all the genes in each predicted multi-component pump. TIGR and SANGER columns show only genes targeted in qRT-PCR. c Pump families assigned based on homology to known (super)family members from other organisms. MFS, major facilitator superfamily; SMR, small multidrug resistance family; ABC, ATP-binding cassette superfamily; RND, resistance-nodulation-cell division superfamily; MATE, multidrug and toxic compound extrusion family; DMT, drug/metabolite transporter superfamily; CDF, cation diffusion facilitator family; ArAE, aromatic acid exporter family; IT, Ion transporter superfamily; HCC, HlyC/CorC family. d Fold change values (FC) are means of triplicates ± standard errors and are presented in decreasing order. For polycistronic transcripts, qRT-PCR was conducted with primers targeting the first pump component-encoding gene of the operon. These genes are marked with a star. nd, transcript not detected in any of the three Y. pestis strains examined (wild-type, Yp pGEM-4Z, and Yp pGEM-RobYp).
