Figure 4
From: Enzymatic, immunological and phylogenetic characterization of Brucella suis urease
Kinetics of the B. suis urease. The graph shows the rectangular hyperbola obtained by nonlinear regression to the initial rate values, which yielded a Km (urea) of 5.60 ± 0.69 mM. The insert graph shows the Lineweaver-Burk plot. Initial rate values are the average of three determinations. Results shown are average ± standard deviation of triplicates of initial rate values for each substrate concentration, using the same enzyme preparation.