Time-course of midgut DENV infection in Ae. aegypti DS3 strain. Immunofluorescent labelling with the specific antibody anti-DEN-2 protein E was followed by confocal microscopy. MGs were dissected from DS3 mosquitoes 5, 13, 26 h and 14 d after infection. Cardia/AMG (panels A, D, G, J), aPMG (panels B, E, H, K), pPMG (panels C, F, I, L).