Processing of predicted substrates of fungal Kex2 proteinases from Candida albicans, C. glabrata, Saccharomyces cerevisiae and Pichia pastoris

Table 2 Compilation of residues relevant for substrate recognition in S. cerevisiae and M. musculus furin.

#	ScKex2	MmFurin	Pocket	Reference
a	D175	D153	S2	[29]
b	D176	D154	S2	[29]
c	D210	D191	S2	[29]
d	D211	N192	S2	[29]
e	Y212	R193	S3' S1'	[31, 32] This work
f	H213	H194	S1'	[32]
g	R216	R197	S3'	[32]
h	I245	M226	S4	[29]
i	L246	L227	S4	[29]
J	I250	V231	S4	[29]
k	T252	D233	S4, S6	[67]
l	D254	V235	S4	[29]
m	E255	E236	S4	[29, 67]
n	W273	W254	S4	[29]
o	A276	E257	S3, S5	[32, 29]
p	D277	D258	S1	[29]
q	D278	D259	S5	[32]
r	H281	T262	S5	[32]
s	Q283	D264	S4, S6	[67]
t	A311	A292	S1	[29]
u	N314	N295	S1	[68]
v	T317	R298	S2'	[31]
w	R318	E299	S2'	[31]
x	D320	D301	S1	[29]
y	D325	D306	S1	[29]
z	Y327	Y308	S4	[31]
A	P347	W328	S2'	[31, 32]
B	Y348	Y329	S2'	[31]
C	E350	E331	S1	[29]
F	Y367	Q350	S4'	This work
G	H369	-	S4'	This work
H	N379	E362	S4'	This work
D	S380	S363	S3' S4'	[31] This work
E	H381	H364	S1'	[32]

Residues relevant for substrate recognition given in the literature or identified in this study were compiled. Column a: Residues were numbered "a" trough "E". The identical numbering was also used in Figure 6 and Figure 7; columns 2 and 3: The respective analogous residues in ScKex2 (Genebank accession no. CAA96143) and MmFurin (Genebank accession no. CAA37988) are given along with the reference.

ISSN: 1471-2180