Figure 1

Plasmid constructions for proteinase expression. (A) Schematic representation of the domain structure of fungal Kex2 proteins. Kex2 consists of a signal peptide, an autocatalytically removed pro-peptide, a catalytic domain, a structural P-domain, a transmembrane domain and finally a cytosolic domain containing sorting signals to the Golgi apparatus. (B) For expression of C. glabrata Kex2 the part of the gene fused with a C-terminal 6 × His tag was cloned into pPic3.5 using the BamHI and NotI restriction sites. (C) For expression of P. pastoris Kex2 the part of the gene fused with a C-terminal 6 × His tag was cloned into pPic3.5 using the SnaBI and NotI restriction sites. (D) For expression of C. albicans Kex2 the part of the gene fused with a C-terminal 6 × His tag was cloned into pCIp10 using the HinDIII and NheI restriction sites.