Figure 5
FACS analysis of cell cycle progression and CPT-induced growth arrest in L. major promastigotes. Dose-response effect of CPT-inducing cellular arrest. Fluorescence intensity representing the cellular DNA content is shown on the X axis, and cell count is shown on the Y axis. A total of 20,000 events were collected in FACS analysis. The left-hand peak in each panel represents G1 cells, the right-hand peak represents G2 cells and the points in between, represent S-phase cells. Exponential growing cultures were treated with the indicated concentrations of CPT for 24 h (T24). After drug removal (by rinsing the cultures in drug-free medium), cells were further incubated in fresh medium for 24 hours (R24) or 48 hours (R48). Cells were collected at each time point, and DNA content was analyzed.