Figure 3

Sensibility of L. major WT (A), the rescued (ΔtopS/ΔtopS+topS) strain (B) and the LmTopIB overexpresing strain (C) to the specific TopIB inhibitor CPT. Cell density was monitored at growing concentrations: (○) control; (▼) 0.125 μM; (●) 0.25 μM; (▽) 0.5 μM; (■) 1 μM; (□) 5 μM and -25 (◇) μM CPT by Coulter. Experiments were carried out by triplicate and error bars represent standard deviations.