Hepatitis B virus genotypes and resistance mutations in patients under long term lamivudine therapy: characterization of genotype G in Brazil

Table 4 Oligonucleotides used in this study

PCR round	Name	Sequence and position	Fragment size (bp)
Nucleotide sequencing of complete genomes
1^st	P1 (sense)	GAAAGCTTGAGCTCTTCTTTTTCACCTCTGCCTAATCA, 1821–841	2241
	S2 (antisense)	GGGTTTAAATGTATACCCAAAGA, 841–819
2^nd	PS1 (sense)	CCATATTCTTGGGAACAAGA, 2826–2845	1236
	S2 (antisense)	GGGTTTAAATGTATACCCAAAGA, 841–819
2^nd	C5 (sense)	AGACCACCAAATGCCCCTATC, 2299–2319	703
	PS3 (antisense)	TCCTTGTTGGGATTGAAGTCCCA, 3002–2980
1^st	PS1 (sense)	CCATATTCTTGGGAACAAGA, 2826–2845	1753
	X3 (antisense)	AGCAGCCATGGAAAGGAGGT, 1383–1363
2^nd	S18 (sense)	GGATGATGTGGTATTGGGGGCCA, 743–765	648
	X3 (antisense)	AGCAGCCATGGAAAGGAGGT, 1383–1363
1^st	S18 (sense)	GGATGATGTGGTATTGGGGGCCA, 743–765	1724
	C2 (antisense)	CTAACATTGAGATTCCCGAGATTGAGA, 2458–2432
2^nd	X1 (sense)	ACCTCCTTTCCATGGCTGCT, 1363–1383	712
	C3 (antisense)	TTGCCTGAGTGCAGTATGGT, 2056–2075
2^nd	PC1 (sense)	GGCTGTAGGCATAAATTGGTCTG, 1781–1803	667
	C2 (antisense)	CTAACATTGAGATTCCCGAGATTGAGA, 2458–2432
Other sequencing and PCR-RFLP experiments
1^st	PS1	CCATATTCTTGGGAACAAGA, 2826–2845	1236
	S2	GGGTTTAAATGTATACCCAAAGA, 841–819
	S22	GTATTTAAATGGATACCCACAGA, 841–819
2^nd	PS1	CCATATTCTTGGGAACAAGA, 2826–2845	1099
	SR	CGAACCACTGAACAAATGGC,704–685
2^nd	S4	TGCTGCTATGCCTCATCTTCT, 416–436	425
	S2	GGGTTTAAATGTATACCCAAAGA, 841–819
	S22	GTATTTAAATGGATACCCACAGA, 841–819

ISSN: 1471-2180