Figure 5

Quantification of the expression of virulence-associated genes of C. jejuni under different conditions. ciaB, dnaJ and racR of C. jejuni strains SC11 and HM5040 were measured in the bacteria incubated under different conditions, A) mock control, B) primary chicken embryo intestinal cells (CEICs) cultured medium, and C) bacteria- and cell-free supernatant from another co-cultivation experiment (see Methods). At 4 h post incubation (p.i.), total bacterial RNA was isolated, and the genes were quantitatively determined by real time quantitative PCR, relative to 16S rRNA. The level of the transcripts of mock control bacteria (A) was defined as 1, to which the level of the genes in other bacteria (B and C) was relative. Data are representative of those from three independent experiments (average ± SD).