Table 4 Bacterial strains and plasmids

C122

prototroph, Δhsd

British Culture Collection Strain No. 122

BL21(DE3)

F^- dcm, ompT hsdS (r_B- m_B-) gal λ (DE3)

[34]

DH10B

F^- – mcrA Δ (mrr-hsdRMS-mcrBC) phi 80lacZ DM15 Δ lacX74 recA1 endA1 ara D139 Δ(ara, leu)7697 galU galK rpsL nupG

Invitrogen

A0 34/86

serotype O83: K24: H31

[9, 10]

DB24

dcm-6 dam-16::Kan mcr-62 Δ(mcrB-hsd-mrr)114

[33]

Plasmids

BAC

pBeloBAC11 cloning vector: ori2 (IncFI), designed for the construction of Bacterial Artificial Chromosomes (BACs), Cm ^R

[35]

BAC C4/1

about 56 kb insert from strain E. coli A0 34/86 (O83: K24: H31) into pBeloBAC11

[11]

pL series

series of pMECA plasmids containing lambda DNA fragments, Ap^R

[22]

pE series

E. coli K-12 chromosomal fragments subcloned into the pUC9 vector, Ap^R

[32]

pEco377I

pMECA plasmid with pEco377I recognition sequence at EcoRV site

[22]

pUC19

cloning vector, Ap^R

[36]

pGC1

hsdR, hsdM and hsdS genes of the EcoEI R-M system, Ap^R

[5]

pFFP30

hsd region of EcoAI on HindIII fragment cloned into pBR322, Ap^R

[5]

pJP24

HincII/NotI fragment from pJP21 carrying the hsdS of the EcoAI R-M system cloned into NdeI/NotI pET32a under control of the P _T7g10 promoter. Ap^R

[37]

pJP21

A derivative of pFFP30 and pET32a carrying the hsdM and hsdS genes of the EcoAI R-M system under control of the P _T7g10 promoter. Ap^R

[37]