Table 2 Primers, their target, sequence, primer pairings and predicted amplicon size of primer pair.
Target | Primer name | Sequence (5' to 3') | Paired primer set and predicted amplicon size |
|---|---|---|---|
Conserved for each IS element | IS1111-1 | ACTGCGTTGGGATACCCATC | NAa |
IS1 | IS1 | TAATGGGCGACCAAGTCGA | IS1111-1; 501 bp |
IS2 | IS2 | GGCTGAATGAATGCCTTCCA | IS1111-1; 501 bp |
IS3 | IS3 | AAAAAGACCCTTCAGGTAATGGAA | IS1111-1; 477 bp |
IS4 | IS4 | TTCCGCCATGACCAACTTC | IS1111-1; 519 bp |
IS5 | IS5 | GTCGGTCAACGTCGTCACAT | IS1111-1; 515 bp |
| Â | 556F | AACCGTGGCTGAGCGATC | IS5; NA |
IS6 | IS6 | CCATTTGCAATAGCCGTGAA | IS1111-1; 477 bp |
IS7 | IS7 | GCGGTTTTGGTACAATACTGTCATT | IS1111-1; 571 bp |
| Â | 1091F | TGCAGGACATATTGTTGATGTCAC | IS7; NA |
IS8 | IS8 | GGCAAGGATGACGAATGGAT | IS1111-1; 471 bp |
IS9 | IS9 | GCCTCAGCCGATTTCGAG | IS1111-1; 492 bp |
| Â | 1219F | CCCGAAGGTATCCATAACTCTGG | IS9; NA |
IS10 | IS10 | GTGCGGCATTCGTGGTAGA | IS1111-1; 481 bp |
| Â | 1269R | GATTTCCATCGACGTCCGC | 1273R; NA |
| Â | 1273R | TTCAGTTTATAGCGGCAGGACC | IS1111-1; NA |
IS11 | IS11 | AAAGCCGTTCCCTACCGTG | IS1111-1; 481 bp |
IS12 | IS12 | ACGTAGTTTGGAAAGGATACCCG | IS1111-1; 482 bp |
IS13 | IS13 | GATGAGCGTCCACCACTGAA | IS1111-1; 537 bp |
IS14 | IS14 | TGCTACCAACAGACTTACGGCA | IS1111-1; 481 bp |
| Â | 1701R | TAAACACGAGGCGCTAGGTCA | IS14; NA |
IS15 | IS15 | AACGCCCATGTGAGAAACATC | IS1111-1; 531 bp |
| Â | 1718F | ACGGGTGTTCAAGCAGCC | IS15; NA |
IS16 | IS16 | ATGACTTCTGATAGGGACTGTGCTC | IS1111-1; 511 bp |
| Â | 1761F | GCCGTTGATCTTGCCGATAA | IS16; NA |
IS17 | IS17 | CGAATCGCATTGGCACAGT | IS1111-1; 477 bp |
IS18 | IS18 | CGTTTGCTGCTATACGACATGAA | IS1111-1; 532 bp |
IS19 | IS19 | GTCGCCCAGGAGTGTTTCTG | IS1111-1; 531 bp |
| Â | 1960R | ACAAAGACGACTCAGTTCGGC | 1963R; NA |
| Â | 1963R | CGATGTCTGCCTTTAGGAGGTC | 1960R; NA |
| Â | 1963R-seq | GTAGAAAGAGTCTCTCTTG | NA |
IS20 | IS20 | ACGTCAATTACATCGAGCATTCA | IS1111-1; 470 bp |
| Â | 1987F | AGGGCCATCTTGACCTGGA | 1993F; NA |
| Â | 1993F | CGCACTGAGCGCATTGAC | 1987F; NA |