Table 3 PCR primers used to create T4ΩRD mutants and the corresponding genes deleted
From: Regions of Diversity 8, 9 and 13 contribute to Streptococcus pneumoniae virulence
| Genes deleted: TIGR annotation | Primers used to amplify flanking DNA fragments for PCR construct deletion mutagenesis† | ||
|---|---|---|---|
| Strain | Upstream fragment | Downstream fragment | |
| T4 ΩRD2 | SP0163-0168 | SP0162 F: NNNNNaagcttGGGATTGGGCTCCTATG | SP0168 F: NNNNNgcggccgcGTACCAACAAATACCTC |
| SP0163 R: NNNNNactagtCTGCTAAAGATAGG | SP0170 R: NNNNNtctagaTCTAACTCTTCATAG | ||
| T4 ΩRD5 | SP0694-0700 | SP0694 F: NNNNNaagcttCCTATCTTGTTGTCATTA | SP0698 F: NNNNNgcggccgcATGGGAACTACTCC |
| SP0694 R: NNNNNactagtCGTAGAACATATTTCGTCC | SP0800 R:NNNNNtctagaACCCAAAACTTCTGC | ||
| T4 ΩRD7 | SP1130-1145 | SP1130 F: NNNNNaagcttCAATTTCTGAAGGTCCG | SP1145 F: NNNNNgcggccgcCATACTGATGATGAGG |
| SP1130 R: NNNNNactagtGTTTAAGCCATGGCG | SP1146 R: NNNNNtctagaCCATAACTAACTCTCC | ||
| T4 ΩRD8 | SP1315-1352 | SP1315 F: NNNNNgcggccgcACTTCGGAAAGAAGTGG | SP1352 F: NNNNNaagcttGTTTCAATGCCCAGCTTCGTCC |
| SP1315 R: NNNNNtctagaCTTCACTTTCATAATACG | SP1352 R: NNNNNactagtCTTTAATGCATCATTAACGACGC | ||
| T4 ΩRD8a | SP1315-1331 | SP1315 F | SP1331 F: NNNNNaagcttTGTTACTGCAAAAAGAAC |
| SP1315 R | SP1331 R: NNNNNactagtGCGCCTTTATCTGCAGC | ||
| T4 ΩRD8b | SP1332-1352 | SP1332 F: NNNNNgcggccgcGAATTTCTTTGTATCGG | SP1352 F |
| SP1332 R: NNNNNtctagaGCAGCAAACTATAAAC | SP1352 R | ||
| T4 ΩRD8b1 | SP1332-1337 | SP1332 F | SP1337 F: NNNNNaagcttCAGTTTACCAAATCATC |
| SP1332 R | SP1337 R: NNNNNactagtCTGCCTCTTCAGAACAATAACG | ||
| T4 ΩRD8b2 | SP1338-1344 | SP1339 F: NNNNNgcggccgcTGCGGTAACTGGAGTG | SP1344 F: NNNNNaagcttTAAAATATACTGGCACGG |
| SP1338 R: NNNNNtctagaTTCTTCTCTACAAGCTC | SP1344 R: CGATGTAGTTactagtGCC | ||
| T4 ΩRD8b3 | SP146-1352 | SP1346 F: NNNNNgcggccgcCCAACAAGTAAATGTCC | SP1352 F |
| SP1346 R:NNNNNtctagaTATAGCGCACCATACC | SP1352 R | ||
| T4 ΩRD9 | SP1615-1621 | SP1615 F: NNNNNaagcttGGGACTTAACTTGAGC | SP1621 F: NNNNNgcggccgcATAGGGAATGTTTACCC |
| SP1615 R: NNNNNactagtCCATTCAGGAGTTGCC | SP1621 R: NNNNNtctagaAAACAAGAAC | ||
| T4 ΩRD12 | SP1950-1957 | SP1950 F: NNNNNaagcttACGAGTGGATTGAC | SP1957 F: NNNNNgcggccgcGATTGAACTTAAACAGG |
| SP1950 R: NNNNNactagtCTTCATAGCTGTGATCCG | SP1957 R: NNNNNtctagaGCCATCTCCCAAATTGCC | ||
| T4 ΩRD13 | SP2158-2166 | SP2158 F: NNNNNaagcttGGAATTCTCAACATAG | SP2166 F: NNNNNgcggccgcCATCTCCTCATCAGG |
| SP2158 R: NNNNNactagtCCACAAATCTTTGC | SP2166 R: NNNNNtctagaCAGATTTGACGAAAGG | ||
