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Table 2 Influence of mismatch position on weighted mismatch calculation

From: Identification of human pathogens isolated from blood using microarray hybridisation and signal pattern recognition

5' – 3' Position

1

2

3

4

N

3

2

1

0.3

0.6

1.0

1.2

1.2

1.1

0.8

0.3

  1. Weights for mismatches related to their position in the sequence. A single mismatch at the first position of the probe is weighted with 0.3 whereas mismatches at central positions of the probes were weighted highest with 1.2. These values were experimentally determined.