Table 1 Frequency of P1 transduction of plsB::kan, plsX::kan, and ygiH::kan mutations into W3110 cells harboring various plasmids
Recipient strain | Relative transduction frequency* | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | Chromosomal gene | Harboring plasmids | P1 donor strain | ||||||||
Strain | plsX | ygiH | pSTV29 | pSTV29 plsB | pSTV28 EcplsX | pSTV29 ygiH | pSTV28 BsplsX | pSTV29 yneS | ΔplsB::kan | ΔplsX::kan | ΔygiH::kan |
W3110 | + | + | - | - | - | - | - | - | 0 | 100 | 100 |
MEC104 | + | + | + | - | - | - | - | - | 0 | 50 | 64.3 |
MEC105 | + | + | - | + | - | - | - | - | 100 | 65.5 | 58.9 |
MEC106 | + | + | - | - | + | - | - | - | 0 | 80 | 50.9 |
MEC107 | + | + | - | - | - | + | - | - | 0 | 100 | 78 |
MEC108 | + | + | - | - | - | - | + | - | 0 | 63.6 | 100 |
MEC109 | + | + | - | - | - | - | - | + | 0 | 81.8 | 70.6 |