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Figure 1 | BMC Microbiology

Figure 1

From: The Yersinia enterocolitica type three secretion chaperone SycO is integrated into the Yop regulatory network and binds to the Yop secretion protein YscM1

Figure 1

Recombinant SycO forms homodimers. (A) Analytical gel filtration shows that SycO elutes from a Superdex 75 column prior to the 29 kDa marker protein, carbonic anhydrase. Consecutive fractions were analyzed by SDS-PAGE (Coomassie-stained); the column was calibrated as indicated above the gel. (B) Chemical cross-linking of recombinant SycO using DSP as cross-linking reagent was analyzed by Coomassie-stained SDS-PAGE.

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BMC Microbiology

ISSN: 1471-2180

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