Figure 8

Detection of the extracellular dsDNA of NgoΦ1 and NgoΦ2 phages of N. gonorrhoeae. PCR amplification was performed on DNA from phage preparation to detect the presence of the DNA sequence corresponding to large terminase encoded by CDS488 (Lane A1) or CDS1116 (Lane A2). In the control experiment the PCR product corresponding to the chromosomal DNA sequence of N. gonorrhoeae FA1090 encoding the lpt3 gene was not detected (Lane A3) in the phage preparation while the same PCR product was formed using the chromosomal DNA as a substrate (Lane B1).