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Figure 5 | BMC Microbiology

Figure 5

From: Characterization of the dsDNA prophage sequences in the genome of Neisseria gonorrhoeae and visualization of productive bacteriophage

Figure 5

Influence of expression of the NgoΦ1 and NgoΦ2 putative promoters on the growth of E. coli Top10 cells as measured by the change of (CFU/ml). The overnight culture of the E. coli Top10 cells carrying cloned CDS479 gene (A) or CDS1116 (B) on the plasmid pMPMK6 grown in LB was diluted 1:50 into 50 ml of the fresh medium and grown at 37°C until the OD650 was equal of about 0.8. At that time the culture was divided and the expression of the cloned gene was started by the addition of the different concentration of arabinose. (A) Symbols: (filled circles), E. coli Top10 (pMPMK6::cds479) not induced; (open squares), E. coli Top10 (pMPMK6::cds479) induced with 0.001% arabinose; (open triangles), E. coli Top10 (pMPMK6::cds479) induced with 0.01% arabinose; (open circles), E. coli Top10 (pMPMK6::cds479) cells induced with 0.1% arabinose; (filled squares), E. coli Top10 (pMPMK6) cells not induced; (open diamonds), E. coli Top10 (pMPMK6) cells induced with 0.1% arabinose. (B) Symbols: (filled circles), E. coli Top10 (pMPMK6::cds1116) not induced; (open squares), E. coli Top10 (pMPMK6::cds1116) induced with 0.001% arabinose; (open triangles) E. coli Top10 (pMPMK6::cds1116) induced with 0.01% arabinose; (open circles) E. coli Top10 (pMPMK6::cds1116) cells induced with 0.1% arabinose. The arrow shows the time when arabinose was added.

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