Regions important for the adhesin activity of Moraxella catarrhalis Hag

Bullard, Brian; Lipski, Serena; Lafontaine, Eric R

doi:10.1186/1471-2180-7-65

BMC Microbiology

Table 1 Strains and plasmids

From: Regions important for the adhesin activity of Moraxella catarrhalis Hag

Strain or plasmid	Description	Source or reference
M. catarrhalis
		[26]
O35E	Wild-type isolate	[26]
O35E.TN2	Isogenic hag mutant strain of O35E	[26]
O12E	Wild-type isolate	[26]
O12E.Hag	Isogenic hag mutant strain of O12E	[26]
TTA37	Wild-type isolate	[26]
TTA37.Hag	Isogenic hag mutant strain of TTA37	[26]
V1171	Wild-type isolate	[26]
V1171.Hag	Isogenic hag mutant strain of V1171	[26]
McGHS1	Wild-type isolate	[26]
McGHS1.Hag	Isogenic hag mutant strain of McGHS1	[26]
E. coli
EPI300	Cloning strain	Epicentre
TUNER	Expression strain	Novagen
Plasmids
pETcoco-1	Protein expression vector, Cm^r	Novagen
pBBCT.77	pETcoco-1 expressing O35E-Hag aa 1358–1964 joined to six N-terminal histidine residues, Cm^r	This study
pCC1.3	Adherence negative plasmid control	[48]
pCC1	Cloning vector, Cm^r	Epicentre
pELO35.Hag	pCC1 expressing the entire O35E hag gene product, Cm^r	[26]
pBBO12.Hag	pCC1 expressing the entire O12E hag gene product, Cm^r	[26]
pSV1171.Hag	pCC1 expressing the entire V1171 hag gene product, Cm^r	[26]
pBBHS2.24	Deletion derivative of pELO35.Hag, missing aa 71–283	This study
pBBHS3.20	Deletion derivative of pELO35.Hag, missing aa 71–384	This study
pBBHS8.18	Deletion derivative of pELO35.Hag, missing aa 71–384 and 1546–1707	This study
pBBHS6.22	Deletion derivative of pELO35.Hag, missing aa 436–1538	This study
pBBHS5.12	Deletion derivative of pELO35.Hag, missing aa 71–283 and 919–1538	This study
pBBHS10.9	Deletion derivative of pELO35.Hag, missing aa 71–384, 746–1193 and 1546–1707	This study
pBBHS10.32	Deletion derivative of pELO35.Hag, missing aa 71–705 and 1546–1707	This study

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ISSN: 1471-2180

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