Immunoblot and fluorescence analyses showing episomal expression of AP33::HA in T. foetus. A and B: Total protein from 107 parasites were separated on 10% SDS -PAGE and blotted on to Hybond-P membrane. The blots were probed with mAb F 5.2 to AP33 (A) and mAb to HA (B). C: Immunofluorescence was performed using anti-HA mAb on non-permeabilized wt (Tf) and transfected trichomonads (Tf-AP33::HA). Brightfield microscopy shows the same trichomonads in the field used for fluorescence microscopy.