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Table 1 Example: matching the M. genitalium spot G18 peptide mass fingerprint data with protein B64230 in the database

From: Mapping phosphoproteins in Mycoplasma genitalium and Mycoplasma pneumoniae

Start-End Observed m/z Mr (expt) Mr (calc) Delta mass(Da) Modification Missed cleavages Database sequence Peptide sequence ion score
4–22 2157.10 2156.09 2156.09 -0.00 Oxidation (M) 0 K.IQVNNIEALNNAMDLALER.D 25
23–41 2070.99 2069.98 2069.98 0.00   0 R.DQNVVLYGQDAGFEGGVFR.A 105
109–118 1074.65 1073.65 1073.62 0.02   0 R.GVYTAPLVVR.M 37
147–157 1268.59 1267.59 1267.58 0.01 Oxidation (M) 0 K.TVMPSNPYDTK.G -
158–176 2090.11 2089.11 2089.11 -0.01   0 K.GLFLAAIESPDPVIFFEPK.K -
158–177 2298.19 2297.19 2297.17 0.01 Phospho (ST) 1 K.GLFLAAIESPDPVIFFEPKK.L -
230–239 1171.69 1170.69 1170.66 0.03   1 K.DKGIELIDLR.T -
232–239 928.57 927.56 927.54 0.02   0 K.GIELIDLR.T 45
247–254 922.51 921.51 921.49 0.01   0 K.QTVFNSVK.K -
259–267 971.63 970.62 970.61 0.01   0 R.LLVVTEAVK.S 42
294–305 1286.77 1285.76 1285.74 0.02   0 R.VTGFDIVVPLAR.G 72
309–316 1040.54 1039.53 1039.51 0.02   0 K.YQFEINAR.V 52
317–326 1112.68 1111.67 1111.66 0.01   0 R.VIDAVNQLLK.- 41
  1. The peptide profile of spot G18, treated with trypsin and analyzed by MALDI-TOF-TOF. Mascot was used for the database search with MSDB version 20050227. 13/45 peptides were matched to B64230, pyruvate dehydrogenase E1-beta chain. The peptide sequence ion score corresponds to peptides analyzed and identified by MS/MS sequencing, and peptides without a score (-) were detected only in the MS data. Ion scores of 20 or greater are significant. The matched peptide sequences are shown by underline in the sequence below.
  2. 1   MSKIQVNNIE ALNNAMDLAL ERDQNVVLYG QDAGFEGGVF R ATKGLQQKY GSERVWDCPI AENSMAGIGV GAAIGGLKPI VEIQFSGFSF PAMFQIFVHA
  3. 101 ARIRNRSRGV YTAPLVVR MP MGGGIKALEH HSETLEAIYA QIAGLKTVMP SNPYDTKGLF LAAIESPDPV IFFEPKK LYR AFRQEIPSDY YTVPIGEANL
  4. 201 ISEGSELTIV SYGPTMFDLI NLVYSGELKD KGIELIDLR T ISPWDKQTVF NSVK KTGRLL VVTEAVK SFT TSAEIITSVT EELFTYLKKA PQRVTGFDIV
  5. 301 VPLAR GEKYQ FEINARVIDA VNQLLK