Figure 2

CHAPS-Soluble Phosphoprotein map for Mycoplasma genitalium. 250 μg CHAPS-soluble cellular extract of stationary phase M. genitalium proteins were separated by 2-D gel electrophoresis with immobilized linear pH gradients from 3 to 10 and 12.5%T SDS-polyacrylamide gels. Panel (A) shows the 2-D gel with the total protein complement stained by SYPRO Ruby. Molecular weight markers are indicated at the left, and pI values at the top. Panel (B) shows the proteins stained by Pro-Q Diamond, and (C) shows the autoradiographic gel obtained by [³³P]-phosphoric acid-labeling. Identified phosphorylated proteins are numbered (see Table 2 for details).