CHAPS-Soluble Phosphoprotein map for Mycoplasma pneumoniae. 250 μg CHAPS-soluble cellular extract of stationary phase M. pneumoniae proteins were separated by 2D PAGE using 12.5%T SDS-polyacrylamide gels. (A) Shows a gel separated in the first dimension using a linear pH gradient p I 3–10 (18 cm IPG strip). Identified phosphorylated proteins are numbered (see Table 3 for details). Molecular weight markers are indicated at the left and pI values at the top. The gel was stained with Pro-Q Diamond for detection of phosphoproteins (shown as blue/white), scanned, and the same gel was then stained with SYPRO Ruby protein stain (shown as red). The overlay images were generated using ImageMaster 2D software.  (B) Shows a partial image of an IPG 6–11 gel stained by Pro-Q Diamond, and (C) shows a partial image from an IPG 4–7 gel stained by Pro-Q Diamond.