Figure 7

Degree of genic sharing of distributed or non-core genes. Dendrogram developed using the unweighted pair group method algorithm demonstrating the degree of genic sharing of distributed or non-core genes [26,28] which are defined as the set of genes not universally present among all strains of the species. The figure compares 15 NTHi strains, which include 9 of the strains phenotyped in the current study (PittDD was omitted due to incomplete genomic data and PittFF and PittGG collapse to a single strain using this method) and the laboratory strain Rd. The sequence for the 86028NP strain has been previously published [18], and the unannotated sequences for the R2866 and R2846 NTHi strains were obtained from Genbank (accession #s NZ_AADP00000000, and NZ_AADO00000000, respectively) and used with permission of the depositing authors. The X-axis lists the number of genic differences between strains; y-axis lists the H. influenzae strains. Strain 86028NP is a nasopharyngeal (NP) isolate obtained from a patient suffering from OM; R2866 is an invasive strain; CHSHi22121 is an NP isolate from a well child; R2846 is an COME isolate; CGSHiR3021 and CGSHi22421 are NP isolates from healthy children; and CHSHi3655 is an OME strain.