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Table 1 Biofilm COMSTAT flow cell measurements for E. coli BW25113 and its isogenic mutants LB glu medium at 24 h and for the dual species (E. coli/P. fluorescens) biofilm in LB medium after 5 days

From: Indole is an inter-species biofilm signal mediated by SdiA

Conditions Strains Biomass, μm3/μm2 Substratum coverage, % Mean thickness, μm Roughness coefficient
Single species of BW25113 and its isogenic mutant BW25113 9 ± 5 8 ± 9 24 ± 8 0.6 ± 0.4
  BW25113 with DMF (control for indole) 6.4 ± 3 6.5 ± 7 21 ± 8 0.5 ± 0.5
  BW25113 with 500 μM indole 4 ± 1.5 18 ± 6 25 ± 6 0.9 ± 0.6
  BW25113 trpE 30 ± 7 13 ± 6 46 ± 3.5 0.3 ± 0.1
Dual species with TOM P. fluorescens 2-79TOM/pHKT3 0.06 ± 0.05 0.90 ± 0.56 0.14 ± 0.13 1.97 ± 0.02
  E. coli K-12 XL1-Blue/pCM18 6.63 ± 2.67 21.52 ± 10.49 13.95 ± 5.67 0.90 ± 0.34
Dual species without TOM P. fluorescens 2-79/pHKT3 0.03 ± 0.01 0.64 ± 0.16 0.04 ± 0.02 1.99 ± 0.01
  E. coli K-12 XL1-Blue/pCM18 0.56 ± 0.51 1.81 ± 1.23 1.04 ± 0.68 1.87 ± 0.06