Table 3 Primer sequence used in PCR.
Genus specific primers (First PCR) | |
|---|---|
Entamoeba genus | E-1 5' TAAGATGCACGAGAGCGAAA 3' (forward primer) E-2 5' GTACAAAGGGCAGGGACGTA 3' (reverse primer) |
Species specific primers (Second nested multiplex PCR) | |
E. histolytica species | EH-1 5' AAGCATTGTTTCTAGATCTGAG 3' (forward primer) EH-2 5' AAGAGGTCTAACCGAAATTAG 3' (reverse primer) |
E. moshkovskii species | Mos-1 5' GAAACCAAGAGTTTCACAAC 3' (forward primer) Mos-2 5' CAATATAAGGCTTGGATGAT 3' (reverse primer) |
E. dispar species | ED-1 5' TCTAATTTCGATTAGAACTCT 3' (forward primer) ED-2 5' TCCCTACCTATTAGACATAGC 3' (reverse primer) |
Internal amplification control (IAC) primer for PCR | |
Human 18S ribosomal RNA gene | IAC-1 5' GGCTTTGGTGACTCTAGATA 3' (forward primer) IAC-2 5' CGTTAAAGGATTTAAAGTGG 3' (reverse primer) |