Table 1 Oligonucleotide sequences used for the amplification and sequencing of nine genetic loci.

gap

glyceraldehydes 3-phosphate dehydrogenase

5' YGCCAAGCGCGTCATCGT 3'

5' GCGGYTGGAGAAGCCCCA 3'

589 bp

AE017223

1685083–1685671

aroA

3-phosphoshikimate 1-carboxyvinyltransferase

5' GACCATCGACGTGCCGGG 3'

5' YCATCAKGCCCATGAATTC 3'

565 bp

AE017223

29974–30538

glk

glucokinase

5' TATGGAAMAGATCGGCGG 3'

5' GGGCCTTGTCCTCGAAGG 3'

475 bp

AE017224

988660–989134

dnaK

chaperone protein

5' CGTCTGGTCGAATATCTGG 3'

5' GCGTTTCAATGCCGAGCGA 3'

470 bp

AE017223

2066742–2067211

gyrB

DNA gyrase B subunit

5' ATGATTTCATCCGATCAGGT 3'

5' CTGTGCCGTTGCATTGTC 3'

469 bp

AE017223

142378–141910

trpE

anthranilate synthase

5' GCGCGCMTGGTATGGCG 3'

5' CKCSCCGCCATAGGCTTC 3'

486 bp

AE017223

1538194–1537709

cobQ

cobyric acid synthase

5' GCGGGTTTCAAATGCTTGGA 3'

5' GGCGTCAATCATGCCAGC 3'

422 bp

AE017223

1289341–1288920

omp25

25 kDa outer-membrane protein

5' ATGCGCACTCTTAAGTCTC 3'

5' GCCSAGGATGTTGTCCGT 3'

490 bp

AE017223

710041–710530

int-hyp

upstream and extreme 5' of hypothetical protein (BruAb1_1395)

5' CAACTACTCTGTTGACCCGA 3'

5' GCAGCATCATAGCGACGGA 3'

430 bp

AE017223

1372708–1372279