β-D-Glucoside utilization by Mycoplasma mycoides subsp. mycoides SC: possible involvement in the control of cytotoxicity towards bovine lung cells

Table 4 Estimated growth of M. mycoides subsp. mycoides SC after treatment with 5 μM sucrose or 5 mM glucose

Pre-treatment of 18 h^a	Ct	Estimated quantity (geq)^b	Culture titre (mycoplasmas/ml)^c	Growth efficacy (%)^d
strain 8740
none	18.26	6.94 × 10⁶	2.77 × 10⁹	100.00
HEPES	25.50	6.12 × 10⁴	2.45 × 10⁷	-0.20
glucose	22.85	3.46 × 10⁵	1.38 × 10⁸	3.94
sucrose	21.07	1.11 × 10⁶	4.42 × 10⁸	15.02
strain T1/44
none	18.13	7.55 × 10⁶	3.02 × 10⁹	100.00
HEPES	24.85	9.35 × 10⁴	3.74 × 10⁷	0.25
glucose	23.57	2.16 × 10⁵	8.63 × 10⁷	1.88
sucrose	24.20	1.43 × 10⁵	5.72 × 10⁷	0.91

^a After pre-treatment with the indicated compound, mycoplasmas were grown for 2 days at 37°C in mycoplasma medium. Compounds: none, mycoplasmas were not subjected to any pre-treatment but were immediately diluted with mycoplasma medium for cultivation of 2 days at 37°C; sucrose/glucose, mycoplasmas were pre-treated for 18 h at 37°C with sucrose or glucose in incubation buffer prior to cultivation for 2 days in mycoplasma medium; HEPES, mycoplasmas were pre-treated with incubation buffer alone.
^b As determined by the formula geq = 1.05 × 10¹² × e^{-0.65 × Ct} generated by the TaqMan standard curve.
^c The culture titre for each test was obtained by multiplying the corresponding geq (amount of total mycoplasmas in 2.5 μl of lysate) by a factor of 400.
^d Calculated by first subtracting the 3 × 10⁷ CFU of departure from each culture titre and considering then as 100% the resulting amount of non-treated mycoplasmas grown in 2-day cultures.

ISSN: 1471-2180