β-D-Glucoside utilization by Mycoplasma mycoides subsp. mycoides SC: possible involvement in the control of cytotoxicity towards bovine lung cells

Table 1 M. mycoides subsp. mycoides SC strains used

Strain^a	Origin	Year Isolated	Host	Bgl SNP^b	Bgl activity^c	Accession number or reference^d
PG1	Unknown	1931	Cattle/type strain	Ala	ND	[10]
B345/93	Portugal	1993	Cattle	Ala	+	AM114901
L2	Italy	1993	Cattle/lung	Ala	+	AM114902
Afadé	Cameroon	1968	Cattle/lung	Val	-	[35]
8740	Cameroon	1987	Cattle	Val	-	AM114903
91130	Central African Republic	1991	Cattle	Val	-	AM114904
T1/44	Tanzania	1952	Cattle/vaccine strain	Ala	+	AM114905
T1/Sr50	Tanzania	1952	Cattle/vaccine strain	Ala	+	[35]
Gladysdale	Australia	Unknown	Cattle	Ala	+	AM114906
DVZ	Australia	1965	Cattle	Ala	+	AM114907

^a Strains were obtained from National Collection of Type Cultures (NCTC), PHLS, London, United Kingdom; Laboratório Nacional de Investigação Veterinária, Lisbon, Portugal; Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland; CIRAD-EMVT, Montpellier, France; and Australian Animal Health Laboratory, Geelong, Victoria, Australia.
^b Residue at amino acid position 204 of Bgl.
^c pNPbG hydrolysis within 1 h (yellow colonies also in the last of the six 10-fold dilutions) corresponds to Bgl activity and is represented by "+"; "-" indicates a reduced enzymatic activity of Bgl (only the areas spotted with the first two 10-fold dilutions became yellowish, and only after 3 h); ND: not done.
^d Sequences of bgl for PG1, Afadé and T1/Sr50 were not determined in this work, as that of PG1 is already available from the genome project [10] and those of Afadé and T1/Sr50 from our previous work [35].

ISSN: 1471-2180