Table 2 Cloning strains and plasmids used in this study
Strain or plasmid | Relevant characteristics | Reference |
|---|---|---|
Strains | Â | Â |
MG1363 | Plasmid-free derivative of L. lactis 712 | Gasson, 1983 |
MG1614 | Streptomycin resistant derivative of MG1363 | Gasson, 1983 |
MG1363 (pMRC01) | MG1363 transconjugant harbouring pMRC01 | Coakley et al., 1997 |
MG1363 (pVE6007) | MG1363 harbouring pVE6007, Cmr | Maguin et al., 1992 |
MG1363 pMRC01 pORI-SOEA pVE6007 | MG1363 harbouring pMRC01, pVE6007 and pORI-SOEA | This study |
MG1363 pMRC01 pORI-SOEB pVE6007 | MG1363 harbouring pMRC01, pVE6007 and pORI-SOEB | This study |
MG1363 pMRC01 pORI-SOEC pVE6007 | MG1363 harbouring pMRC01, pVE6007 and pORI-SOEC | This study |
MG1363 pMRC01 pORI-SOEA | MG1363 harbouring pORI-SOEA integrated into pMRC01 | This study |
MG1363 pMRC01 pORI-SOEB | MG1363 harbouring pORI-SOEB integrated into pMRC01 | This study |
MG1363 pMRC01 pORI-SOEC | MG1363 harbouring pORI-SOEC integrated into pMRC01 | This study |
Mutant A | MG1363 transconjugant harbouring pMRC01sacαAsacβA ΔltnA1A2 | This study |
LtnA1/Sacα Hybrid | MG1363 transconjugant harbouring pMRC01ltnA1leadersacαAΔltnA1 (sacαA is fused to ltnA1 leader) | This study |
LtnA2/Sacβ Hybrid | MG1363 transconjugant harbouring pMRC01ltnA2leadersacβAΔltnA2 (sacβA is fused to ltnA2 leader) | This study |
E. coli EC101 | E. coli JM101 with repA from pWV01 integrated into the chromosome | Law et al., 1995 |
E. coli EC101 pORI280 | E. coli EC101 harbouring pORI280 | Leenhouts et al., 1996 |
E. coli EC101 pORI-ltnA1A2 | E. coli EC101 harbouring pORI280-ltnA1A2 | Cotter et al., 2005 |
E. coli pORI-N15K | E. coli EC101 harbouring pORI-N15K | This study |
E. coli pORI-A17N | E. coli EC101 harbouring pORI-A17N | This study |
E. coli pORI-L21A | E. coli EC101 harbouring pORI-L21A | This study |
MG1363 pMRC01 pVE6007 pORI-N15K | MG1363 harbouring pMRC01, pVE6007 and pORI-N15K | This study |
MG1363 pMRC01 pVE6007 pORI-A17N | MG1363 harbouring pMRC01, pVE6007 and pORI-A17N | This study |
MG1363 pMRC01 pVE6007 pORI-L21A | MG1363 harbouring pMRC01, pVE6007 and pORI-L21A | This study |
MG1363 pMRC01 pORI-N15K | MG1363 harbouring pORI-N15K integrated into pMRC01 | This study |
MG1363 pMRC01 pORI-A17N | MG1363 harbouring pORI-A17N integrated into pMRC01 | This study |
MG1363 pMRC01 pORI-L21A | MG1363 harbouring pORI-L21A integrated into pMRC01 | This study |
LtnA1-N15K | MG1363 harbouring pMRC01 LtnA1-N15K | This study |
LtnA1-A17N | MG1363 harbouring pMRC01 LtnA1-A17N | This study |
LtnA1-L21A | MG1363 harbouring pMRC01 LtnA1-L21A | This study |
Plasmids | Â | Â |
pORI280 | RepA- ori+ β-galactosidase-positive Eryr integration vector | Leenhouts et al., 1996 |
pORI-SOEA | ltnsacαAsacβAltn containing derivative of pORI280 | This study |
pORI-SOEB | ltnA1leadersacαAltn containing derivative of pORI280 | This study |
pORI-SOEC | ltnA2leadersacβAltn containing derivative of pORI280 | This study |
pVE6007 | RepA+, Cmr, temperature sensitive derivative of pWV01 | Maguin et al., 1992 |
pORI280-ltnA1A2 | ltnA1A2 containing derivative of pORI280 | Cotter et al., 2005 |
pORI-N15K | ltnA1 site directed mutant N15K derivative of pORI280 | This study |
pORI-A17N | ltnA1 site directed mutant A17N derivative of pORI280 | This study |
pORI-L21A | ltnA1 site directed mutant L21A derivative of pORI280 | This study |