Comparison of commercial and in-house Real-time PCR assays for quantification of Epstein-Barr virus (EBV) DNA in plasma

Table 1 Intra- and inter-assay reproducibility of EBV RQ-PCR in-house assay and Q-EBV PCR quantification kit tested on serial dilutions of culture supernatant.

Comparison	Method	Dilution	Mean EBV DNA load (copies/reaction)	ST. DEV.	CV (%)
INTRA-ASSAY	EBV RQ-PCR	Und.	19511	2557	13
		1:10	5403	420	8
		1:100	2055	605	29
	Q-EBV PCR	Und.	1852	312	17
		1:10	75	12	16
		1:100	12	4	36
INTER-ASSAY	EBV RQ-PCR	Und.	43774	16467	28
		1:10	2406	544	35
		1:100	1116	618	37
	Q-EBV PCR	Und.	4363	998	21
		1:10	241	84	24
		1:100	22	7	31

ISSN: 1471-2180