Impact of the rpoS genotype for acid resistance patterns of pathogenic and probiotic Escherichia coli

BMC Microbiology

Table 2 Oligonucleotide primers used in this study.

Target gene	Name	Position	5' Sequence 3'	⇔
rpoS	RpoS 3	4 – 24	GCT CTA GA A GTC AGA ATA CGC TGA AAG TT Xba I	s
rpoS	RpoS 4	989 – 969	CCG AGC TC C TCG CGG AAC AGC GCT TCG AT Sac I	as
rpoS	RpoS 4c	1129 – 1109	CCC AAG CTT TTA CTC GCG GAA CAG CGC TTC Hind III	as
rpoS	RpoS 5	572 – 589	ATG AAC CAA GTG GG AAG	s
rpoS	RpoS 6	871 – 854	ACA TCT TCC AGT GTT GCC	as
nlpD	RpoS 8	(- 657) – (- 636)	CCC AAG CTT CGA CGC AGC AGA GCA AGG AGT T Hind III	s
rpoS	RpoS 9	1141 – 1119	CCC AAG CTT CAG CTG GTG CGC AAG ATG ATG AA Hind III	as
rpoS	RpoS 17a	706 – 736	GAC ATC CTG GCC GAT G AA AAA GAG AAC GGT C	s
rpoS	RpoS 17b	736 – 706	GAC CGT TCT CTT TTT C AT CGG CCA GGA TGT C	as
rpoS	RpoS 22	(+ 171) – (+ 151)	CCC AAG CTT GCC AAA TGT GAC GCT GAC GCG Hind III	as

Primers used in this study are listed with their 5' – 3' sequences. All nucleotide positions refer to the start codon of their target gene. Positions of RpoS primers within rpoS, nlpD or the flanking regions are referenced to the open reading frame of rpoS. Positions 5' upstream of the start codon are indicated with a negative sign, positions 3' downstream of the stop codon with a positive one. s = sense, as = antisense

ISSN: 1471-2180