Diagram representing the AP65-3 recombinant clones. Clones c1 through c5 represent individual recombinant fragments cloned in pET26b(+) vector (Novagen EMD Chemicals Inc) using primers indicated in Table 1. Each recombinant fragment contains a C-terminal His·Tag® used for purification. The AP65 protein is illustrated above the clones, and numbers indicate the relative positions of amino acids. R is the relative position of rabbit antiserum reactive epitopes, and M and M' refer to epitopes reactive with mAbs 12G4 and F11, respectively, as determined before .