Table 2 Bacterial strains and plasmids
Bacterial strain or plasmid | Relevant feature(s) | Reference or source |
|---|---|---|
Strains | Â | Â |
B. holmesii | Â | Â |
ATCC 515 41 | clinical isolate | [19] |
No1 | clinical isolate | [23] |
G8341 | clinical isolate | [19] |
G7702 | clinical isolate | [19] |
G7702 bvgA | G7702 with a kanamycin-resistance cassette disrupting the bvgA gene | [27] |
B. pertussis | Â | Â |
Tohama I (TI) | wildtype, but rpsL | [43] |
BP359 | derivative of TI, bvgA::Tn5 | [43] |
E. coli | Â | Â |
DH5α | strain used for high-efficiency transformation | Gibco |
SM10 | mobilizing strain | [38] |
Plasmids | Â | Â |
PbluescriptSK | high copy number cloning vector | Stratagene |
pMMB208 | Broad-host-range expression vector | [44] |
pKEN2 | plasmid containing the promoterless gfp-mut2 gene | [40] |
pSK-FP | pSK carrying a 312 bp PCR fragment of B. holmesii G7702 derived from the upstream region of fhaB | This study |
pSK-fhaP-gfp0 | pSK carrying a fusion between 277 bp of the fhaB promoter region of B. holmesii G7702 harbouring the putative bindings sites BS1–BS4 and the gfp-mut2 gene | This study |
pMMB208-fhaP-gfp0 | pMMB208 carrying a fusion between 277 bp of the fhaB promoter region of B. holmesii G7702 (BS1–BS4) and the gfp-mut2 gene | This study |
pMMB208-fhaP-gfp2 | pMMB208 carrying a fusion between 224 bp of the fhaB promoter region of B. holmesii G7702 (BS2–BS4) and the gfp-mut2 gene | This study |
pMMB208-fhaP-gfp3 | pMMB208 carrying a fusion between 168 bp of the fhaB promoter region of B. holmesii G7702 (BS3–BS4) and the gfp-mut2 gene | This study |
pMMB208-fhaP-gfp4 | pMMB208 carrying a fusion between 146 bp of the fhaB promoter region of B. holmesii G7702 (BS4) and the gfp-mut2 gene | This study |
pMMB208-fhaP-gfp6 | pMMB208 carrying a fusion between 111 bp of the fhaB promoter region of B. holmesii G7702 and the gfp-mut2 gene | This study |