Skip to main content

Table 2 Bacterial strains and plasmids

From: Identification and regulation of expression of a gene encoding a filamentous hemagglutinin-related protein in Bordetella holmesii

Bacterial strain or plasmid Relevant feature(s) Reference or source
Strains   
B. holmesii   
ATCC 515 41 clinical isolate [19]
No1 clinical isolate [23]
G8341 clinical isolate [19]
G7702 clinical isolate [19]
G7702 bvgA G7702 with a kanamycin-resistance cassette disrupting the bvgA gene [27]
B. pertussis   
Tohama I (TI) wildtype, but rpsL [43]
BP359 derivative of TI, bvgA::Tn5 [43]
E. coli   
DH5α strain used for high-efficiency transformation Gibco
SM10 mobilizing strain [38]
Plasmids   
PbluescriptSK high copy number cloning vector Stratagene
pMMB208 Broad-host-range expression vector [44]
pKEN2 plasmid containing the promoterless gfp-mut2 gene [40]
pSK-FP pSK carrying a 312 bp PCR fragment of B. holmesii G7702 derived from the upstream region of fhaB This study
pSK-fhaP-gfp0 pSK carrying a fusion between 277 bp of the fhaB promoter region of B. holmesii G7702 harbouring the putative bindings sites BS1–BS4 and the gfp-mut2 gene This study
pMMB208-fhaP-gfp0 pMMB208 carrying a fusion between 277 bp of the fhaB promoter region of B. holmesii G7702 (BS1–BS4) and the gfp-mut2 gene This study
pMMB208-fhaP-gfp2 pMMB208 carrying a fusion between 224 bp of the fhaB promoter region of B. holmesii G7702 (BS2–BS4) and the gfp-mut2 gene This study
pMMB208-fhaP-gfp3 pMMB208 carrying a fusion between 168 bp of the fhaB promoter region of B. holmesii G7702 (BS3–BS4) and the gfp-mut2 gene This study
pMMB208-fhaP-gfp4 pMMB208 carrying a fusion between 146 bp of the fhaB promoter region of B. holmesii G7702 (BS4) and the gfp-mut2 gene This study
pMMB208-fhaP-gfp6 pMMB208 carrying a fusion between 111 bp of the fhaB promoter region of B. holmesii G7702 and the gfp-mut2 gene This study