Determination of transcriptional start sites of the gfp reporter gene fused to the upstream region of fhaB of B. holmesii by primer extension analysis. Equal amounts of total RNA extracted from B. holmesii G7702 (pMMB208-fhaP-gfp0) (lane 1) and B. holmesii G7702 bvgA (pMMB208-fhaP-gfp0) (lane 2) were hybridized with radiolabelled oligonucleotide gfp.PE. The cDNAs corresponding to the transcripts P1 to P3 are indicated by arrows. A part of the fhaB promoter sequence is shown on the left. Transcriptional start points are indicated by arrows. The sequencing reaction (lanes A, C, G and T) was performed using oligonucleotide gfp.PE and plasmid pSK-fhaP-gfp0 as a template.