Table 2 Primers used in this study*.
From: No detectable effect of RNA-binding protein Hfq absence in Staphylococcus aureus
Name | Position | Sequence | Use |
|---|---|---|---|
SA-hfq 5up (BamH1) | F | 5'CGGGATCC ATGACGATTCTTTAGGTGA3' | Used for cloning upstream region of hfq |
SA-hfq 5down (KpnI) | R | 5'GGGGTACC TGTCGGACTCCTTTTACT3' | Â |
SA-hfq 3up (SmaI) | F | 5'TCCCCCGGG ATGGGCACGATTTAATGAC3' | Used for cloning downstream region of hfq |
SA-hfq 3down (EcoRI) | R | 5'CGGAATTC TACTTGAAACACCTGATT | Â |
SA-cat 5 (KpnI) | F | 5'GGGGTACC GCACAGACAGGACAAAATCG | Used for cat amplification |
SA-cat 3 (SmaI) | R | 5'TCCCCCGGG TTCCGAGGCTCAACGTC3' | Â |
SA-hfq verif up | F | 5'ATCATAGCAGGTGGAACAG3' | Used for verification of hfq deletion |
SA-hfq verif down | R | 5'CGAAAGAGAAATAGAAAAAT3' | Â |
SA-RNAIII 5 | F | 5'CAGAGATGTGATGGAAAATAGTTG3' | Used for RNA III probe amplification |
SA-RNAIII 3 | R | 5'ATTAAGGGAATGTTTTACAGTTATT3' | Â |
SA-spa 5 | F | 5'TTAGCATCTGCATGGTTTGC3' | Used for spa probe amplification |
SA-spa 3 | R | 5'ACAACGTAACGGCTTCATCC3' | Â |
SA-hu 5 | F | 5'AGATTTAATCAATGCAGTTGCAGA3' | Used for hu probe amplification |
SA-hu 3 | R | 5'AATGCTTTACCAGCTTTG AATGCT3' | Â |
SA-hfq 5 (NdeI) | F | 5'GGAATTCCATATG ATTGCAAACGAAAAC3' | Used for hfq probe amplification, RT-PCR |
SA-hfq 3 (HindIII) | R | 5'CCCAAGCTT ATTCTTCACTTTCAGTAG3' | Â |