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Table 1 Strains and plasmids used in this study.

From: No detectable effect of RNA-binding protein Hfq absence in Staphylococcus aureus

  Key features Reference
Strains   
E. coli DH5αZ1 Δ(argF-lac)169 ϕ80Δlac Z58(M15) gln V44 (AS) rfb D1 gyr A96 (NalR) rec A1 end A1 spo T1 thi-1 hsdR1 7 Z1(lacR tetR SpR) [36]
S. aureus RN4220 Avirulent mutant of NCTC 8325-4 that accepts foreign DNA [43]
S. aureus RN6390 Laboratory virulent strain derived from NCTC 8325 [43]
S. aureus COL Highly methicillin resistant clinical isolate [44]
S. aureus Newman Virulent strain containing a high level of Clumping Factor [45]
S. aureus RN4220 hfq RN4220, Δhfq::cat, CmR This work
S. aureus RN6390 hfq RN6390, Δhfq::cat, CmR This work
S. aureus COL hfq COL, Δhfq::cat, CmR This work
S. aureus Newman hfq Newman, Δhfq::cat, CmR This work
Plasmids   
PMAD Thermosensitive plasmid in S. aureus derived from pRN5101 and containing the bgaB gene, EryR, AmpR [38]
pMADΔhfq::cat pMAD containing up- and downstream regions from hfq gene cloned on either side of the Cm cassette (cat); used for construction of the S. aureus RN4220 hfq mutant, EryR, AmpR, CmR, bgaB This work