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Table 1 Determination of DHS activity from P. vivax by eIF-5A modification

From: Cloning, expression and functional activity of deoxyhypusine synthase from Plasmodium vivax

incubation temp. 37°C incubation temp. 37°C incubation temp. 37°C
eIF5A lys Water 50 μM GC7
14[-C]-spermidine 14[-C]-spermidine 14[-C]-spermidine
1268 U DHS/mg protein n.d. U DHS/mg protein 35 U DHS/mg protein
P. vivax DHS P. vivax DHS P. vivax DHS
A B C
  1. column A) complete enzymatic assay B) control: DHS enzyme was substituted by water C) inhibition of DHS by the inhibitor GC7 (50 μM).