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Table 1 Determination of DHS activity from P. vivax by eIF-5A modification

From: Cloning, expression and functional activity of deoxyhypusine synthase from Plasmodium vivax

incubation temp. 37°C

incubation temp. 37°C

incubation temp. 37°C

eIF5A lys

Water

50 μM GC7

14[-C]-spermidine

14[-C]-spermidine

14[-C]-spermidine

1268 U DHS/mg protein

n.d. U DHS/mg protein

35 U DHS/mg protein

P. vivax DHS

P. vivax DHS

P. vivax DHS

A

B

C

  1. column A) complete enzymatic assay B) control: DHS enzyme was substituted by water C) inhibition of DHS by the inhibitor GC7 (50 μM).
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BMC Microbiology

ISSN: 1471-2180

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