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Table 3 Confirmation of clinical samples of bovine faeces, positive for MAP with the primary PCR.

From: New PCR systems to confirm real-time PCR detection of Mycobacterium avium subsp. paratuberculosis

Samples

n

Confirmed*

Additional 5 replicates†

Additional 20 replicates‡

F57 failure§

Culture-confirmed

47

41

4

2

0

Not culture-confirmed

9

3

5

0

1

  1. * Confirmed by all three alternative systems, using two PCR replicates.
  2. † Confirmed by DH3 on F57, only after running 5 additional PCR replicates.
  3. ‡ Confirmed by DH3 on F57, only after running 20 additional PCR replicates.
  4. §Confirmation with the DH3 system failed after a total of 22 PCR replicates
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BMC Microbiology

ISSN: 1471-2180

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