Skip to main content

Table 3 Confirmation of clinical samples of bovine faeces, positive for MAP with the primary PCR.

From: New PCR systems to confirm real-time PCR detection of Mycobacterium avium subsp. paratuberculosis

Samples n Confirmed* Additional 5 replicates† Additional 20 replicates‡ F57 failure§
Culture-confirmed 47 41 4 2 0
Not culture-confirmed 9 3 5 0 1
  1. * Confirmed by all three alternative systems, using two PCR replicates.
  2. † Confirmed by DH3 on F57, only after running 5 additional PCR replicates.
  3. ‡ Confirmed by DH3 on F57, only after running 20 additional PCR replicates.
  4. §Confirmation with the DH3 system failed after a total of 22 PCR replicates