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Table 3 Invasion and intracellular survival of selected Schu S4 mutants in J774.11

From: Identification of transposon insertion mutants of Francisella tularensis tularensis strain Schu S4 deficient in intracellular replication in the hepatic cell line HepG2

Strain

No. of intracellular CFUs recovered from cells at 24 hrs.

SCHU S4

3.00 × 107 ± 0.346

BJM1025 (hflB)

1.80 × 107 ± 0.200 p < 0.0065*

BJM1004 (MFS)

1.35 × 107 ± 0.0866 p < 0.0013*

BJM1005 (dsbB)

8.33 × 105 ± 0.0577

BJM1026 (hypothetical)

2.32 × 105 ± 1.29

BJM1001 (pyrB)

1.80 × 108 ± 0.0643

BJM1002 (aroA)

2.18 × 108 ± 0.387

BJM1003 (yfiO)

1.17 × 108 ± 0.135

BJM10012 (carA)

1.14 × 108 ± 0.867

BJM10015 (purL)

4.03 × 107 ± 0.802

BJM10018 (purH)

2.70 × 107 ± 0.100

BJM1024 (Na+/H+ transporter)

1.12 × 108 ± 0.872

  1. 1J774.1 cells were infected with bacteria at an MOI to 50:1 After 2 hrs the media was replaced with media containing gentamicin. Sets of three wells were lysed 24 hrs later, diluted, and spread on MHA plates to determine the number of intracellular colony forming units (CFUs). Each time point represents the average of three wells.
  2. * Compared to Schu S4
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BMC Microbiology

ISSN: 1471-2180

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