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Table 3 Invasion and intracellular survival of selected Schu S4 mutants in J774.11

From: Identification of transposon insertion mutants of Francisella tularensis tularensis strain Schu S4 deficient in intracellular replication in the hepatic cell line HepG2

Strain No. of intracellular CFUs recovered from cells at 24 hrs.
SCHU S4 3.00 × 107 ± 0.346
BJM1025 (hflB) 1.80 × 107 ± 0.200 p < 0.0065*
BJM1004 (MFS) 1.35 × 107 ± 0.0866 p < 0.0013*
BJM1005 (dsbB) 8.33 × 105 ± 0.0577
BJM1026 (hypothetical) 2.32 × 105 ± 1.29
BJM1001 (pyrB) 1.80 × 108 ± 0.0643
BJM1002 (aroA) 2.18 × 108 ± 0.387
BJM1003 (yfiO) 1.17 × 108 ± 0.135
BJM10012 (carA) 1.14 × 108 ± 0.867
BJM10015 (purL) 4.03 × 107 ± 0.802
BJM10018 (purH) 2.70 × 107 ± 0.100
BJM1024 (Na+/H+ transporter) 1.12 × 108 ± 0.872
  1. 1J774.1 cells were infected with bacteria at an MOI to 50:1 After 2 hrs the media was replaced with media containing gentamicin. Sets of three wells were lysed 24 hrs later, diluted, and spread on MHA plates to determine the number of intracellular colony forming units (CFUs). Each time point represents the average of three wells.
  2. * Compared to Schu S4