Table 3 Primers used in this study
From: Efflux pump genes of the resistance-nodulation-division family in Burkholderia cenocepacia genome
Primer | Sequence of primers | Comments | Product size (bp) |
|---|---|---|---|
2pbadFOR | 5'-CACCATGGCCAAGTTCTTTATC-3' | Sense primer for the cloning of orf2-coding into pBAD202 | 3192 |
2pbadREV | 5'-TTACTGCCCCTTCGAATCGTTT-3' | Antiense primer for the cloning of orf2-coding into pBAD202 | Â |
1rtFOR | 5'-CCACGCCGTTCGTTCGCATCT-3' | Sense primer for RT-PCR analysis of orf1 | 577 |
1rtREV | 5'-GCTTGGCGAACCACCGGTTGA-3' | Antisense primer for RT-PCR analysis orf1 | Â |
2rtFOR | 5'-CGAAGCTGAAGCCGTTGAC-3' | Sense primer for RT-PCR analysis orf2 | 511 |
2rtREV | 5'-TCGTGCTGTCGGCGGTGTT-3' | Antisense primer for RT-PCR analysis orf2 | Â |
3rtFOR | 5'-CGCGCTCACCGACCTGATGTT-3' | Sense primer for RT-PCR analysis orf3 | 531 |
3rtREV | 5'-AGCGCGAGGAACACGACGAGC-3' | Antisense primer for RT-PCR analysis orf3 | Â |
4rtFOR | 5'-AACATCCTGCGCTGAAGGTCAAC-3' | Sense primer for RT-PCR analysis orf4 | 537 |
4rtREV | 5'-TGACAGCCCCTCTTCCGCCATCAC-3' | Antisense primer for RT-PCR analysis orf4 | Â |
5rtFOR | 5'-TACGTGCTGCGCGAACTGAAG-3' | Sense primer for RT-PCR analysis orf5 | 594 |
5rtREV | 5'-AACAGCCACATCACGCAAAACAC-3' | Antisense primer for RT-PCR analysis orf5 | Â |
6rtFOR | 5'-TCGAATGGGTGCTGAACCGC-3' | Sense primer for RT-PCR analysis orf6 | 477 |
6rtREV | 5'-CGAATTCTTCCGAGTTGGGGCT-3' | Antisense primer for RT-PCR analysis orf6 | Â |
8rtFOR | 5'-CAAGGTCGTGACCGGCGTGTA-3' | Sense primer for RT-PCR analysis orf8 | 517 |
8rtREV | 5'-GATCATCGACAGCACCATCAGC-3' | Antisense primer for RT-PCR analysis orf8 | Â |
9rtFOR | 5'-CTATTCGATCAATACGCTCACG-3' | Sense primer for RT-PCR analysis orf9 | 493 |
9rtREV | 5'-CGCATAGACGGCGAACACCAC-3' | Antisense primer for RT-PCR analysis orf9 | Â |
10rtFOR | 5'-AACTCGCTGCAGATCTCGGAC-3' | Sense primer for RT-PCR analysis orf10 | 521 |
10rtREV | 5'-ATCGCGAACTGCTTGTAGAACTG-3' | Antisense primer for RT-PCR analysis orf10 | Â |
11rtFOR | 5'-TCGCGGCGAAGATGGAGGAC-3' | Sense primer for RT-PCR analysis orf11 | 522 |
11rtREV | 5'-TCGTCGCCATCGGATGGAACAT-3' | Antisense primer for RT-PCR analysis orf11 | Â |
12rtFOR | 5'-CGCGCTCAACGACTGGTTCCT-3' | Sense primer for RT-PCR analysis orf12 | 520 |
12rtREV | 5'-CAGTTGCGATCGGTCGTAGGTC-3' | Antisense primer for RT-PCR analysis orf12 | Â |
13rtFOR | 5'-CAGGTTACACAGATGCGAACGA-3' | Sense primer for RT-PCR analysis orf13 | 530 |
13rtREV | 5'-AAGCCCGACACGTGACGAGCG-3' | Antisense primer for RT-PCR analysis orf13 | Â |
14rtFOR | 5'-ACGCCGACCGACATCTTTCCG-3' | Sense primer for RT-PCR analysis orf14 | 615 |
14rtREV | 5'-CTGCGTGCTGCCGTTCATCTGC-3' | Antisense primer for RT-PCR analysis orf14 | Â |
15rtFOR | 5'-AGAGTTTGATCATGGCTCAG-3' | Sense primer for RT-PCR analysis rRNA 16S gene | 1490 |
16rtREV | 5'-TACCTTGTTACGACTTCAC-3' | Antisense primer for RT-PCR analysis rRNA 16S gene | Â |