Figure 3
From: A novel flow cytometric assay for measurement of In Vivo pulmonary neutrophil phagocytosis
Determination of percentage of PMNs with internalized bacteria. For each day of the assay, unlabeled S. pneumoniae were instilled into the lungs of a single rat. The PMNs collected from that rat by BAL [the RP-1 (PE) positive cells] were analyzed for autofluorescence in the CSFE channel and used to set a marker (shown in A) to delineate the PMNs in all other samples that had phagocytosed CFSE-labeled bacteria (shown in B).