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Table 1 Recombinant ALO ED50 in the absence of serum. Results show amount of rALO necessary for 50% cytolysis of primary human PMNs, lymphocytes, monocyte-derived macrophages, THP-1 monocytes, ME-180, D562, and A549 epithelial cells after 30 min at 37°C. * PMN cytolysis was measured at 10 min. Data derived from Figures 1–5 by cytotoxicity curve extrapolation.

From: The Bacillus anthracis cholesterol-dependent cytolysin, Anthrolysin O, kills human neutrophils, monocytes and macrophages

  rALO ED50
  ng/ml nM
PMNs* 4 0.070
THP-1 monocytes 20 0.35
Lymphocytes 25 0.44
MDMs 100 1.8
ME-180 1000 18
D562 1000 18
A549 50 0.88