Figure 7
Bacillus anthracis strains secreting ALO lyse THP-1 monocytes in a dose and time dependent manner. Percent viable THP-1 s in the presence of B. anthracis Sterne strain (A.)7702 (ALO+), (B.) UT231 (ALO-) and (C.) the complemented over-producer, UT231 (pUTE544) (ALO++) at MOIs of 1000, 100, 10 and 1 in serum free conditions. At time points, aliquots were mixed with 0.25 % trypan blue. Results are average ± standard deviations of three experiments, each performed in duplicate. (MOI of 100, 30 min, 1 h, 2 h, p < 0.001,1 way ANOVA. p < 0.001 for UT231/7702, UT231/UT231 (pUTE544), 7702/UT231 (pUTE544), Tukey test. MOI of 10, 30 min, 1 h, 2 h, p < 0.001,1 way ANOVA. 30 min, p < 0.001 for UT231/UT231 (pUTE544) and 7702/UT231 (pUTE544), Tukey test. 1 h and 2 h, p < 0.001 for UT231/7702, UT231/UT231 (pUTE544), 7702/UT231 (pUTE544), Tukey test.