Antifungal drug time-kill assay. The rates of survival of strains 8656, wdpks1 Δ-1, and wdpks1 Δ-1-501 yeast cells grown for 7 days in YPD medium at 37°C after exposure to various concentrations of amphotericin B [A] or voriconazole [B] for 2 h compared to those of fungal cells incubated in PBS. Values are averages and brackets represent standard errors of the means for three measurements. P values were calculated by comparing wild-type and wdpks1 Δ-1 cells using the Student's t test. *, p < 0.01. Similar results were obtained in three separate experiments.