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Table 5 Regulation of E. coli and Y. pestis dppA::lacZ translational gene fusions by the Y. pestis gcvB gene. Cells were grown in LB (E. coli) or in HIB (Y. pestis) at 37°C to an OD600 ~0.5 and assayed for β-galactosidase activity [16]. Activity is expressed in Miller units. The parent strains KIM6 and KIM6ΔgcvB grown in HIB at 37°C showed <5 units of β-galactosidase activity.

From: The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules

Lysogen Relevant genotype β-Galactosidase activity
GS162λdppAEc::lacZ WT 103 ± 24
GS1144λdppAEc::lacZ ΔgcvB 554 ± 81
GS1144λdppAEc::lacZ[pgcvBYp-p322] ΔgcvB/gcvBYp 154 ± 32
KIM6[pdppAYp::lacZ] WT 62 ± 13
KIM6ΔgcvB[pdppAYp::lacZ] ΔgcvB 455 ± 7