Table 1 Bacteria, bacteriophage and plasmids used in this study.

Strains

E.coli

EC101

Cloning host, Kan^R.

[48]

ER2683

F'proA+B+ lacIq Δ lacZM15 miniTn10 (Kan^R) fhuA2 Δ(lacI-lacA)200glnV44e14-rfbD1? relA1? endA1 spoT1?thi-1 Δ(mcrC-mrr)114::IS10.

NEB collection*

M15

Expression host for pQE 60 derivatives.

Qiagen

L.Lactis

MG1614

Plasmid-free derivative of L.Lactis supsp. lactis 712

[49]

NZ9000

MG1363, pepN::nisRK, nisin inducible overexpression host.

[42]

NCK690KP1

Partially cured derivative of NCK203. Host strain for phage Ul36

[50]

Bacteriophage

λ

E.coli temperate bacteriophage.

NEB collection*

Sk1

Small isometric headed phage for MG1614.

[51]

C2

Prolate headed phage for MG1614.

[52]

Ul36

P335 lytic phage for NCK690 KP1.

[53]

Plasmids

pPTPi

Low-copy number E.coli-L.Lactis shuttle vector for cloning, Tc^R.

[1]

pJO-J

pPTPi derivative containing complete 6.2 kb LlaJI operon from pNP40.

[1]

pJO-M1M2

pPTPi derivative containing 3.0 kb fragment from pNP40 encompassing promoter, llaJIM1 and llaJIM2 genes.

[1]

pNZ8020

High-copy number E.coli-L.Lactis overexpression vector, PnisA, Cm^R.

[54]

pNZ-R1

pNZ8020 derivative containing llaJIR1 under control of PnisA.

This study

PNZ-R2

pNZ8020 derivative containing llaJIR2 under control of PnisA.

This study

pNZ-R1R12

pNZ8020 derivative containing llaJIR1 and llaJIR2 under control of PnisA.

This study

pUC19

High-copy number E.coli cloning vector, Amp^R.

[55]

pUC-R1

pUC19 derivative containing llaJIR1.

This study

pUC-R2

pUC19 derivative containing llaJIR2.

This study

pUC-R1R2

pUC19 derivative containing llaJIR1 and llaJIR2.

This study

pMal-c2x

Medium-copy number expression plasmid

NEB collection*

pMal-R2

pMal-c2x derivative containing llaJIR2.

This study

pQE60

High-number 6xhis tag expression plasmid.

Qiagen

pQE-R1

pQE60 derivative containing llaJIR1.

This study

pQE-R2

pQE60 derivative containing llaJIR2.

This study

pRep4

Low-copy number LacI expressing pQE60 companion

Qiagen

plasmid.