|
Strains
| | |
|
E.coli
| | |
|
EC101
|
Cloning host, KanR.
|
[48]
|
|
ER2683
|
F'proA+B+ lacIq Δ lacZM15 miniTn10 (KanR) fhuA2 Δ(lacI-lacA)200glnV44e14-rfbD1? relA1? endA1 spoT1?thi-1 Δ(mcrC-mrr)114::IS10.
|
NEB collection*
|
|
M15
|
Expression host for pQE 60 derivatives.
|
Qiagen
|
|
L.Lactis
| | |
|
MG1614
|
Plasmid-free derivative of L.Lactis supsp. lactis 712
|
[49]
|
|
NZ9000
|
MG1363, pepN::nisRK, nisin inducible overexpression host.
|
[42]
|
|
NCK690KP1
|
Partially cured derivative of NCK203. Host strain for phage Ul36
|
[50]
|
|
Bacteriophage
| | |
|
λ
|
E.coli temperate bacteriophage.
|
NEB collection*
|
|
Sk1
|
Small isometric headed phage for MG1614.
|
[51]
|
|
C2
|
Prolate headed phage for MG1614.
|
[52]
|
|
Ul36
|
P335 lytic phage for NCK690 KP1.
|
[53]
|
|
Plasmids
| | |
|
pPTPi
|
Low-copy number E.coli-L.Lactis shuttle vector for cloning, TcR.
|
[1]
|
|
pJO-J
|
pPTPi derivative containing complete 6.2 kb LlaJI operon from pNP40.
|
[1]
|
|
pJO-M1M2
|
pPTPi derivative containing 3.0 kb fragment from pNP40 encompassing promoter, llaJIM1 and llaJIM2 genes.
|
[1]
|
|
pNZ8020
|
High-copy number E.coli-L.Lactis overexpression vector, PnisA, CmR.
|
[54]
|
|
pNZ-R1
|
pNZ8020 derivative containing llaJIR1 under control of PnisA.
|
This study
|
|
PNZ-R2
|
pNZ8020 derivative containing llaJIR2 under control of PnisA.
|
This study
|
|
pNZ-R1R12
|
pNZ8020 derivative containing llaJIR1 and llaJIR2 under control of PnisA.
|
This study
|
|
pUC19
|
High-copy number E.coli cloning vector, AmpR.
|
[55]
|
|
pUC-R1
|
pUC19 derivative containing llaJIR1.
|
This study
|
|
pUC-R2
|
pUC19 derivative containing llaJIR2.
|
This study
|
|
pUC-R1R2
|
pUC19 derivative containing llaJIR1 and llaJIR2.
|
This study
|
|
pMal-c2x
|
Medium-copy number expression plasmid
|
NEB collection*
|
|
pMal-R2
|
pMal-c2x derivative containing llaJIR2.
|
This study
|
|
pQE60
|
High-number 6xhis tag expression plasmid.
|
Qiagen
|
|
pQE-R1
|
pQE60 derivative containing llaJIR1.
|
This study
|
|
pQE-R2
|
pQE60 derivative containing llaJIR2.
|
This study
|
|
pRep4
|
Low-copy number LacI expressing pQE60 companion
|
Qiagen
|
| |
plasmid.
| |
