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Table 1 Bacteria, bacteriophage and plasmids used in this study.

From: A genetic dissection of the LlaJI restriction cassette reveals insights on a novel bacteriophage resistance system

Strains and Plasmids Revevant characteristic(s) Reference or source
Strains   
E.coli   
EC101 Cloning host, KanR. [48]
ER2683 F'proA+B+ lacIq Δ lacZM15 miniTn10 (KanR) fhuA2 Δ(lacI-lacA)200glnV44e14-rfbD1? relA1? endA1 spoT1?thi-1 Δ(mcrC-mrr)114::IS10. NEB collection*
M15 Expression host for pQE 60 derivatives. Qiagen
L.Lactis   
MG1614 Plasmid-free derivative of L.Lactis supsp. lactis 712 [49]
NZ9000 MG1363, pepN::nisRK, nisin inducible overexpression host. [42]
NCK690KP1 Partially cured derivative of NCK203. Host strain for phage Ul36 [50]
Bacteriophage   
λ E.coli temperate bacteriophage. NEB collection*
Sk1 Small isometric headed phage for MG1614. [51]
C2 Prolate headed phage for MG1614. [52]
Ul36 P335 lytic phage for NCK690 KP1. [53]
Plasmids   
pPTPi Low-copy number E.coli-L.Lactis shuttle vector for cloning, TcR. [1]
pJO-J pPTPi derivative containing complete 6.2 kb LlaJI operon from pNP40. [1]
pJO-M1M2 pPTPi derivative containing 3.0 kb fragment from pNP40 encompassing promoter, llaJIM1 and llaJIM2 genes. [1]
pNZ8020 High-copy number E.coli-L.Lactis overexpression vector, PnisA, CmR. [54]
pNZ-R1 pNZ8020 derivative containing llaJIR1 under control of PnisA. This study
PNZ-R2 pNZ8020 derivative containing llaJIR2 under control of PnisA. This study
pNZ-R1R12 pNZ8020 derivative containing llaJIR1 and llaJIR2 under control of PnisA. This study
pUC19 High-copy number E.coli cloning vector, AmpR. [55]
pUC-R1 pUC19 derivative containing llaJIR1. This study
pUC-R2 pUC19 derivative containing llaJIR2. This study
pUC-R1R2 pUC19 derivative containing llaJIR1 and llaJIR2. This study
pMal-c2x Medium-copy number expression plasmid NEB collection*
pMal-R2 pMal-c2x derivative containing llaJIR2. This study
pQE60 High-number 6xhis tag expression plasmid. Qiagen
pQE-R1 pQE60 derivative containing llaJIR1. This study
pQE-R2 pQE60 derivative containing llaJIR2. This study
pRep4 Low-copy number LacI expressing pQE60 companion Qiagen
  plasmid.  
  1. * New England Biolabs collection, obtained from M. Sibley and E. Raleigh at NEB.