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Table 1 Purification of mutacin H-29B by hydrophobic chromatography.

From: Mutacin H-29B is identical to mutacin II (J-T8)

Step Volume (mL) Activity (AU/mL) Total Protein (mg) Total activity (AU. 103) Specific activity (AU/mg) Yield (%) Purification (fold)
Culture supernatant 1250 6400 10000 8000 800 100 1
XAD-7 Amberlite 300 6400 97.5 1920 20000 24 25
Sep-Pak C18 95 12800 1.9 1216 6.4 × 105 15.2 800
C18 RP-HPLC 1 6.4 × 105 0.1 640 6.4 × 106 8 8000