Table 1 Purification of mutacin H-29B by hydrophobic chromatography.
Step | Volume (mL) | Activity (AU/mL) | Total Protein (mg) | Total activity (AU. 103) | Specific activity (AU/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|---|---|
Culture supernatant | 1250 | 6400 | 10000 | 8000 | 800 | 100 | 1 |
XAD-7 Amberlite | 300 | 6400 | 97.5 | 1920 | 20000 | 24 | 25 |
Sep-Pak C18 | 95 | 12800 | 1.9 | 1216 | 6.4 × 105 | 15.2 | 800 |
C18 RP-HPLC | 1 | 6.4 × 105 | 0.1 | 640 | 6.4 × 106 | 8 | 8000 |