From: The Bacillus anthracis chromosome contains four conserved, excision-proficient, putative prophages
 |  |  | Primer | PCR product | Location |
---|---|---|---|---|---|
Primer Name | ORF/gene product | Sequence 5' to 3' | Length | Size (bp) | Â |
ORF01192Fa | BA0479-hypothetical | AAACCCTGGGACCTCTGAAC | 20 | 1002 | prophage 04 |
ORF01192Ra | BA0479-hypothetical | GGAAGAATCGCACGACCATC | 20 | Â | prophage 04 |
ORF02190Fa | BA5356-Terminase, large subunit | GACATCGTTGCACCTTCACAAG | 22 | 1462 | prophage 03 |
ORF02190Ra | BA5356-Terminase, large subunit | CCAAATGTCGAGCATCTTGTTC | 22 | Â | prophage 03 |
ORF03655 Fb | BA4094-Terminase, large subunit | TTGATCGATCCATCTCCTGAAC | 22 | 1192 | prophage 02 |
ORF03655 Rb | BA4094-Terminase, large subunit | GATCAACTTTAGCCTGCGGAAC | 22 | Â | prophage 02 |
Ba02 #7 Forc | BA4094-Terminase, large subunit | CAAAGACAGATCCACCTGGAC | 21 | 304 | prophage 02 |
Ba02#7 Revc | BA4094-Terminase, large subunit | CAAAGGGAGGTTCAGCATCTC | 21 | Â | prophage 02 |
ORF03991 Fa | BA3805-Acyl transferase | AGTTGCATGCCCAGTTCTTG | 20 | 1205 | prophage 01 |
ORF03991 Ra | BA3805-Acyl transferase | CTGCGTGACTGGAATCCCTTAC | 22 | Â | prophage 01 |
Ba04INF | LambdaBa04 left junction | CCAGTTGAATCCAGAACAAACG | 22 | 871 | prophage 04 |
Ba04OUTR | LambdaBa04 left junction | GGGCAGTCATACGAGGATAATG | 22 | (961,865)d | prophage 04 |
Ba04OUTF | LambdaBa04 right junction | CCTTCGCTTTGAATTCCTTCTC | 22 | 997 | prophage 04 |
Ba04INR | LambdaBa04 left junction | GAATTGTAACGAGCATGGAAGC | 22 | Â | prophage 04 |
Ba03INF | LambdaBa03 left junction | ACGTTACCCCTATTTCCGAAGC | 22 | 938 | prophage 03 |
Ba03OUTR | LambdaBa03 left junction | CATTTTAATGCGCCCACGAC | 20 | (923, 952)d | prophage 03 |
Ba03OUTF | LambdaBa03 right junction | TTCCACATCTTCCTTCAGCAAC | 22 | 977 | prophage 03 |
Ba03INR | LambdaBa03 left junction | GGCCGTACTGGCTTAACTTCTG | 22 | Â | prophage 03 |
Ba02INF | LambdaBa02 left junction | TCACTTGCCAGTCTTGACCTTG | 22 | 891 | prophage 02 |
Ba02OUTR | LambdaBa02 left junction | GGTGCATAAGGCGGTAAAGATG | 22 | (861,962)d | prophage 02 |
Ba02OUTF | LambdaBa02 right junction | GGCGAGGTATTAGCTTTACAGTGG | 24 | 976 | prophage 02 |
Ba02INR | LambdaBa02 left junction | GTCCATCTTCACTGCCGAAAC | 21 | Â | prophage 02 |
Ba011NF | LambdaBa01 left junction | ACAAATTCAGTTGCGCTTCC | 20 | 1053 | prophage 01 |
Ba010UTR | LambdaBa01 left junction | TGCAGCACCTACACTGAAACAAG | 23 | (878, 1047)d | prophage 01 |
Ba010UTF | LambdaBa01 right junction | CGATGGAAAGTTCTTACCGAAG | 22 | 915 | prophage 01 |
Ba011NR | LambdaBa01 left junction | ATGATGCTCGTCACTTCATCG | 21 | Â | prophage 01 |
gmk F | BC guanylate kinase, putative | ATTTAAGTGAGGAAGGGTAGG | 21 | 500 | chr |
gmk R | BC guanylate kinase, putative | GCAATGTTCACCAACCACAA | 20 | Â | chr |
PA1 For | Protective antigen | ATCACCAGAGGCAAGACACC | 20 | 311 | pXO1 |
PA1 Rev | Protective antigen | CCATTGTTTCAGCCCAAGTT | 20 | Â | pXO1 |
bla F | Amp resistance | TTACCAATGCTTAATCAGTGAGGC | 24 | 861 | plasmid |
bla R | Amp resistance | ATGAGTATTCAACATTTCCGTGTC | 24 | Â | plasmid |
Primers Used in Real Time PCR for determination of Phage Excision Frequencies | Â | Â | Â | Â | Â |
Bce_glpR | BC glycerol kinase | GCAGTAGCGGTTGCAGCATA | 20 | 150 | chr |
Bce_glpF | BC glycerol kinase | CCCGATAATTGCCCCAATC | 19 | Â | chr |
Ba04circF | LambdaBa04 circle junction | TCAAACCCATCAACAATTTCATTG | 24 | 129 | Extra-chr |
Ba04circR | LambdaBa04circle junction | CAATAGAATGCACAACAGTGACATAAGT | 28 | Â | Extra-chr |
Ba03circF | LambdaBa03 circle junction | CAAGGGTTGTAGCTGATAGCTCATT | 25 | 170 | Extra-chr |
Ba03circR | LambdaBa03 circle junction | TGACAAAGTTCAGTCGATTTTTTTCT | 26 | Â | Extra-chr |
Ba02circR | LambdaBa02 circle junction | GACCACAACTTGTACCACATTTATTATTT | 29 | 166 | Extra-chr |
Ba02circF | LambdaBa02 circle junction | CCGCAATATAGGTGGTATAATGCA | 24 | Â | Extra-chr |
Ba01circF | LambdaBa01 circle junction | CCCACAAAATAAAAAAACCCTCAA | 24 | 150 | Extra-chr |
Ba01circR | LambdaBa01 circle junction | ACGTTTTTGGCGCAATTTAAA | 21 | Â | Extra-chr |
Ba04mtF | LambdaBa04 deleted junction | AGCACGTGATGTACAAGCGTTAA | 23 | 150 | φ free chr |
Ba04mtR | LambdaBa04 deleted junction | TATTCCCTCATATCATGAGGGAATATG | 27 |  | φ free chr |
Ba03mtF | LambdaBa03 deleted junction | TGGCCAAATCAAAACTGGTTCT | 22 | 165 | φ free chr |
Ba03mtR | LambdaBa03 deleted junction | TTCTCACGGTCTGTCGTTATTTTC | 24 |  | φ free chr |
Ba02mtF | LambdaBa02 deleted junction | ATATCACCTCAAGGCAACAAACAA | 24 | 150 | φ free chr |
Ba02mtR | LambdaBa02 deleted junction | GGTAATCTCTCCTTTCGATGTAGCA | 25 |  | φ free chr |
Ba01mtF | LambdaBa01 deleted junction | CATTAGGAGATCACTTACTTGAGCACTT | 28 | 161 | φ free chr |
Ba01mtR | LambdaBa01 deleted junction | CACAAATAAAAAAACCTTGATACCGTAGT | 29 |  | φ free chr |
Primers Used in Real time PCR screening of Prophage Genes | Â | Â | Primer | PCR product | Location |
Ba04-RT-F | BA0479-hypothetical | TATAATGGGCACTCCATTTTGGT | 23 | 150 | prophage 04 |
Ba04-RT-R | BA0479-hypothetical | TCCACAGTGGCATTTACCTTTG | 22 | Â | prophage 04 |
Ba03-RT-F | BA5356-Terminase, large subunit | TCCTATCGAGAATGGGTTCAACTAT | 25 | 150 | prophage 03 |
Ba03-RT-R | BA5356-Terminase, large subunit | GCGTCCCTACCGTTCAACTG | 20 | Â | prophage 03 |
Ba02-RT-F | BA4094-Terminase, large subunit | TGCGTTTTATCATGGAAAATGC | 22 | 150 | prophage 02 |
Ba02-RT-R | BA4094-Terminase, large subunit | TGAGCCAGGTGCTCGTGTT | 19 | Â | prophage 02 |
Ba01-RT-F | BA3805-Acyl transferase | CACTTGAATCAACTGGTATCGTGAA | 25 | 150 | prophage 01 |
Ba01-RT-R | BA3805-Acyl transferase | AAATCCAAATTGAGGCATATGATGA | 25 | Â | prophage 01 |